This study evaluates the effect of temperature on thermal comfort and energy conservation by utilizing the previously used local ventilation air-conditioning mode. Three room temperatures (26°C, 28°C, 30°C (78.8°F, 82.4°F, 86°F)) and three LV temperatures (20°C, 26°C, 28°C (68°F, 78.8°F, 82.4°F)) were used. Eighteen subjects participated in the subjective experiment where they reported thermal sensation and thermal comfort. In addition, the energy consumption of a cooling system was calculated. The conditions of 26°Crt and 20°Clv (78.8°Frt and 68°Flv) (the room temperature of 26°C (78.8°F) combined with the LV temperature of 20°C (68°F)) and 30°Crt and 26°Clv (86°Frt and 78.8°Flv) were found to be in the comfortable range. The relationship between the thermal comfort improvement and energy conservation was indicated. The condition of 28°Crt and 26°Clv (82.4°Frt and 78.8°Flv) was assessed to be the most comfortable environment with moderate energy consumption. Compared with the traditional air conditioning mode, which creates a uniform environment by setting the room temperature to be 24°C (75.2°F), the utilization of LV could increase the comfortable room temperature to 30°C (86°F) and consume 24% less energy.